Jared Lee Loughner made his second appearance in court since the January 8 shooting spree that left six people dead and entered a plea of not guilty on Monday.

The 22-year-old faces charges of the attempted assassination of US Representative Gabrielle Giffords and the attepmpted murder of her two aides; he will be charged for the other killings in state court.

PHOTOS: Jared Lee Loughner Is The Suspected Shooter

Loughner’s hair has grown out and his sinister “smirk was back on his face,” according to CNN.  He entered the courtroom in Phoenix, Arizona 10 minutes before the judge came into the room and was looking around, smiling and chuckling a little bit.

Authorities recovered nearly two dozen surveillance tapes from both inside and outside the Arizona Safeway grocery store where Jared Lee Loughner went on a wild shooting spree, and the footage is graphic and disturbing.

Sources who have viewed the tapes told the Washington Post that Loughner is seen shooting Rep. Gabrielle Giffords in the face, above the left eye, from just two or three feet away.

PHOTOS: The Six People Killed In The Tucson Shootings

“He walks up, goes bang. It’s very clear,” a source said.

“He is maybe 24 to 36 inches away. You can see her go straight down.”

The family of Jared Lee Loughner has not been seen publicly since their son allegedly opened fire at a political event outside a grocery story on Saturday morning that wounded 14 people and took the lives of six others, but in a statement released Tuesday, they expressed their sorrow for the victims.

“This is a very difficult time for us.  We ask the media to respect our privacy. There are no words that can possibly express how we feel.  We wish that there were, so that we could make you feel better.  We don’t understand why this happened. It may not make any difference, but we wish that we could change the heinous events of Saturday.  We care very deeply about the victims and their families. We are so very sorry for their loss. Thank you.”

PHOTOS: The Six Arizona Shooting Victims

The statement was handed to the media waiting outside the Loughner’s home in Tucson, Arizona. As RadarOnline.com previously reported, the FBI were initially denied access to the home Tuesday but eventually went inside to speak with the family.

Deranged shooter Jared Loughner’s family blocked FBI agents from gaining entry to their home, RadarOnline.com has learned.

The family - who have still not made a public statement about the shooting in which six people died - put on 4-by-4 double-thick plywood that blocked access to the front porch of their north Tucson home.

PHOTOS: The Six People Killed In The Arizona Shooting

At about 12:25 p.m., agents began banging on the blockade, yelling, "This is the FBI. Let us in."

Several FBI agents were then seen going around the back of the house before they were allowed access via the front of the property.

Police have just released this mugshot to RadarOnline.com... showing Arizona shooting suspect Jared Lee Loughner smirking just moments after he was taken into custody.

The alleged murderer is accused of gunning down 20 people - including Congresswoman Gabrielle Giffords - in a supermarket parking lot this weekend.

PHOTOS: The Six People Killed In The Arizona Shooting

And this disturbing mugshot, taken shortly after police stopped his rampage, will leave the American public with chills.

With his shaved head, crazy looking eyes and smug grin, Loughner looks like a mad man.

PHOTOS: Tucson Massacre Crime Scene

Arizona shooter Jared Loughner had an "evil smile on his face like The Joker from Batman", his college math teacher told RadarOnline.com in an exclusive new interview Monday.

Ben McGahee -- who taught Loughner at Pima Community College last summer -- believed that the 22-year-old was high on drugs in his class and represented a threat to his other students.

McGahee was so alarmed about Loughner's behavior that he alerted the college Dean and counselors when he discovered that Loughner had scribbled the words ‘Mayhem Fest’ on one of his math tests.

The man accused of killing six people and wounding 14 others, including Congresswoman Gabrielle Giffords, appeared in a Phoenix federal courtroom on Monday where he answered questions and a judge set a future court date.

With his head shaved, Jared Lee Loughner entered the Arizona courtroom in shackles and wearing a tan prison uniform, CNN reported.  His eyes were "darting back and forth," but he spoke in a "strong voice" when questioned by the judge.

PHOTOS: The Six People Killed In The Arizona Shooting

When the proceedings began, the judge asked him if he was Jared Lee Loughner and he replied "Yes, I am Jared Lee Loughner."

Loughner's family was not present in the courtroom.